Cell Culture Passage Planner

🧫 Virtual Cell Culture Passage Planner

Plan your passage – calculate transfer volumes, media, and track population doublings.

               1-Current Culture

               2-Passage Plan

               3-Results & Protocol

Step 1: Describe your current culture

Cell concentration (cells/mL)

Current culture volume (mL)

Viability (%)

Current passage number

Current vessel type T25 flask (25 cm²)T75 flask (75 cm²) T175 flask (175 cm²)6-well plate (9.6 cm²) 12-well plate (3.8 cm²)24-well plate (1.9 cm²) 96-well plate (0.32 cm²)Custom area

Custom surface area (cm²)

Step 2: Define your passage parameters

How do you want to specify the seeding density?

Target seeding density (cells/cm²) Split ratio (e.g., 1:5)

Seeding density (cells/cm²)

Destination vessel T25 flask (25 cm²)T75 flask (75 cm²) T175 flask (175 cm²)6-well plate (9.6 cm²) 12-well plate (3.8 cm²)24-well plate (1.9 cm²) 96-well plate (0.32 cm²)Custom area

Destination custom area (cm²)

Split ratio (e.g., 5 for 1:5)

Destination vessel T25 flask (25 cm²)T75 flask (75 cm²) T175 flask (175 cm²)6-well plate (9.6 cm²) 12-well plate (3.8 cm²)24-well plate (1.9 cm²) 96-well plate (0.32 cm²)Custom area

Destination custom area (cm²)

Doubling time (hours, optional)

Culture duration after seeding (hours)

Step 3: Your Passage Plan

📋 Step‑by‑Step Protocol